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Brissard, Charline; Herrenknecht, Christine; Sechet, Veronique; Herve, Fabienne; Pisapia, Francesco; Harcouet, Jocelyn; Lemee, Rodolphe; Chomerat, Nicolas; Hess, Philipp; Amzil, Zouher. |
Ostreopsis cf. ovata produces palytoxin analogues including ovatoxins (OVTXs) and a putative palytoxin (p-PLTX), which can accumulate in marine organisms and may possibly lead to food intoxication. However, purified ovatoxins are not widely available and their toxicities are still unknown. The aim of this study was to improve understanding of the ecophysiology of Ostreopsis cf. ovata and its toxin production as well as to optimize the purification process for ovatoxin. During Ostreopsis blooms in 2011 and 2012 in Villefranche-sur-Mer (France, NW Mediterranean Sea), microalgae epiphytic cells and marine organisms were collected and analyzed both by LC-MS/MS and hemolysis assay. Results obtained with these two methods were comparable, suggesting ovatoxins... |
Tipo: Text |
Palavras-chave: Ostreopsis cf. ovata; Palytoxin & ovatoxins; Culture; Seafood contamination; LC-MS/MS; Hemolysis assay; Mediterranean Sea. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31025/29428.pdf |
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Brissard, Charline. |
Dinoflagellates of genus Ostreopsis are known in tropical areas to provoke food intoxication, after palytoxin accumulation in seafood. Probably in link with climatic changes, Ostreopsis sp. is increasingly present in French Mediterranean since 2006. In the context of the PhD, the studies in natural environment, in summer 2011 and 2012, showed that: i) Ostreopsis cf. ovata strain presented a complex toxinic profile, including palytoxin and especially ovatoxins (OVTX-a, -b, c, -d, -e). A new analog (OVTX-h) was detected; ii) cell and toxinic concentrations decreased with depth; iii) different seafood accumulated toxins, which could represent real risk for bathers and consumers. Because there are no standards for OVTXs, one of the PhD goals was to develop a... |
Tipo: Text |
Palavras-chave: Ostreopsis ovata; Ovatoxines; Purification; Contamination des produits de la mer; Chromatographie liquide/spectrométrie de masse.; Ostreopsis ovata; Ovatoxins; Purification; Seafood contamination; Liquid chromatography / mass spectrometry.. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00255/36613/35179.pdf |
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